DNA Fragmentation Analysis

What is it?

Sperm DNA fragmentation analysis is a test that helps to assess the percentage of sperm that has damaged DNA. The sperm DNA fragmentation analysis can be done in conjunction with a semen analysis to provide a more complete picture of sperm health and reproductive capability.

Why is it done?

Due to oxidative stress sperm is particularly susceptible to damage, which can cause ‘breaks’ in the DNA strands. Certain individuals have a much higher risk of this damage occurring, especially if they are:

• Regular smokers,
• Obese,
• Of advanced age, or
• Regularly exposed to toxic substances

DNA fragmentation has been directly linked with male infertility, therefore a DNA fragmentation analysis can help to identify the cause and allow steps to be taken to improve fertility outcomes.

How do I arrange a semen analysis and sperm DNA fragmentation?

To arrange a semen and sperm DNA fragmentation analysis you can get a referral from your GP. Please contact Concept Fertility at (08) 9382 2388 to book a suitable time. A sample can be collected between 8am and 2pm Monday to Friday.

What is involved?

1. Semen sample for analysis are produced by masturbation and collected in a sterile specimen jar.
2. Concept Fertility has special rooms within the clinic that are designed for collection of a sample. If you prefer, you can chose to produce your sample from home. This sample must be delivered to the clinic within the hour. It is essential that you avoid exposing the sperm to extreme temperature and sunlight. For couples that have religious convictions, special condoms which are not toxic to sperm can be purchased at Concept Fertility.
3. We will also review the information collected from your questionnaire to help interpret the results of your semen analysis.
4. There are many ways to test DNA damage. At Concept Fertility, the Halosperm assay is used. During this process, sperm that are normal will appear with a ‘halo’ and DNA damaged sperm will appear without (refer to image below. The sperm with arrows are considered to have DNA damage.)

 

How much does the test cost?

Please refer to our Fee Schedule.

How are the results interpreted?

The results are interpreted as per the below:

• <20% - a low level result
• 21-29% - a moderate result
• >30% - a severe level of DNA fragmentation. 

The results of this test will be used by your doctor in conjunction with you and your partner's other assessments to develop your treatment plan. You can receive your results from the doctor that referred you for the test. While a normal semen analysis is reported within 7 days, this is a specialised test which takes longer to process. Results are usually available within 2-3 weeks. Please keep this in mind before booking your follow-up doctor’s appointment.

Depending on your results, one of our fertility specialists will provide a further explanation and determine which treatment option is right for you. 
 

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